Broadcasting legend Sue, 66, who has fronted tennis for the BBC for nearly three decades, was surprised on air with a touching tribute video.
It included praise and thanks from a host of tennis stars, including Andy Murray, Roger Federer and Rafael Nadal.
Martina Navratilova, Tim Henman and Pete Sampras also supplied contributions.
“I think it is fair to say we’ve broken her,” Clare Balding later joked as nearby attendees chanted Sue’s name.
Sue Barker steps away from BBC Wimbledon coverage
Commentator John McEnroe surprised Sue on air as he introduced a highlight package showing her as a player and anchor.
And among the many fond words said about Sue, Andy Murray said: “I’ve grown up watching you on the TV.
“And then obviously been interviewed by you here many times.
“You’re going to be sadly missed by everyone who loves and watches tennis.”
Billie Jean King has referred to Sue as “the GOAT” on several occasions. The former number one US star added: “She has lived it. She understands it.
“Sue understands the highs and lows of Wimbledon as a player.”
The incredible Sue Barker. The best in the business. 👏
— BBC Sport (@BBCSport) July 11, 2022
How Sue reacted
An overwhelmed Sue – who reached the semi-finals of Wimbledon in 1977 as a player – said her BBC run was “an absolute privilege”.
Meanwhile, she added: “All I’ll say is I’ll miss the job, I wish I had the next 30 years to do. I love it.
“But most of all I’m going to miss the people I work with.”
Sue, who according to reports turned down a three-year contract extension by the BBC, continued: “In front of the camera, behind the camera, you’ve just been absolutely amazing.
I’ve been so proud to the front the programme, so thank you.
“I’ve been so proud to the front the programme, so thank you.”
Tributes from fans on Twitter
Fans also paid fulsome tributes to Sue on Twitter.
“She’s an absolute institution and will be missed,” one social media user wrote.
“Congratulations to Sue on a wonderful career, sporting and presenting! I hope she enjoys a happy retirement.”
Meanwhile another tweeter posted: “Sue Barker will be missed by us all.
“Wimbledon coverage won’t be the same without her knowledge, humour, and natural flair for presenting.
“The end of an era and big boots to fill!”
In addition, a third tweeted: “She’s brilliant. Loved watching her. Like they say she’s the GOAT of presenting.”
Leave us a comment on our Facebook page @EntertainmentDailyFix and let us know what you think of this story.